Unlike other urea sensing methods reported so far, using this urea aptamer, we demonstrate a simple, ''non-enzymatic'' easy-to-use, dual readout aptasensor that exploits unmodified gold nanoparticles (AuNPs) to transduce the signals of aptamer binding to urea in terms of intrinsic fluorescence differences and color changes simultaneously.
· Number of times cited according to CrossRef: 96. Colloidal Nanoparticles for Biomedical Applications XIV San Francisco, United States Colloidal Nanoparticles for Biomedical Applications XIV SPIE, (2019).9781510624269 9781510624276 Angela Federica De Fazio, Jack Haines, Alex Courtier, Otto Muskens and Antonios G. Kanaras Optical response of gold and upconversion nanoparticles assembled via DNA ...
Afterwards a silver deposition on the bound gold nanoparticles generates a conductive layer between the electrodes. The measured drop in the resistance serves as signal for the chip-based electrical detection of DNA 3 . To further optimize this system the gold nanoparticles as seed are replaced by the enzyme horseradish peroxidase.
We report a DNA-gold nanoparticle (AuNP) hybrid hydrogel in which the AuNPs crosslink enzymatically synthesized DNA to form a gel network. PCR-elongated DNA and AuNPs act as a one-dimensional polymer and cross-linkers, respectively. The DNA-AuNP hydrogel has the functional properties of both long DNA and the AuNPs.
comparitive overview of DNA detection, using enzyme-assisted, gold nanoparticles-based transducing platforms see Table SI. 1. Herein, we present an innovative sensing technology based on the unique enzymatic activity of RNAse H for the detection of bacterial DNA at concentrations down to …
· In the present study, a novel and facile method is introduced for the sensitive detection of the M.SssI methyltransferase (M.SssI MTase) enzyme and methylated DNA based on the fluorescence recovery of FAM-labeled DNA coupled with gold nanoparticles …
· Processing and characterization of gold nanoparticles for use in plasmon probe spectroscopy and microscopy of biosystems. Chen Y, Preece JA, Palmer RE. Ann N Y Acad Sci, 1130:201-206, 01 Jan 2008 Cited by: 6 articles | PMID: 18596349. Review
· Gold nanoparticle-labeled detection antibodies for use in an enhanced electrochemical immunoassay of hepatitis B surface antigen in human serum. Mikrochim Acta. 2009;166:269-75 77. Ding C, Li H, Hu K, Lin J-M. Electrochemical immunoassay of hepatitis B surface antigen by the amplification of gold nanoparticles based on the nanoporous gold ...
The development of eco-friendly technologies in material synthesis is of considerable importance to expand their biological applications. Nowadays, a variety of inorganic nanoparticles with well-defined chemical composition, size, and morphology have been synthesized by using different microorganisms, and their applications in many cutting-edge technological areas have been explored.
· In addition, gold is generally well tolerated by the human body and has the advantage of linking easily to DNA. The CRISPR-Gold system—which consists of a DNA-linked gold nanoparticle containing Cas9, guide RNA, and a DNA repair template—is designed to enter cells through endocytosis, a process in which the cell engulfs outside molecules.
Gold Nanoparticles labeled DNA ; Gold Nanoparticles labeled RNA ; Gold Nanoparticles labeled Streptavidin ... their applications in food processing and tissue engineering are particularly disadvantageous because many of these compounds are rated as toxic or may form harmful by-products leached from the cross-linked protein matrix ...
DNA assembly and enzymatic cutting in solutions: A gold nanoparticle based SERS detection strategy Elizabeth Crew, Hong Yan, Liqin Lin, Jun Yin, Zakiya Skeete, Timur Kotlyar, Nuri Tchah, Jehwan Lee, Michael Bellavia, Isaac Goodshaw, Pharrah Joseph, Jin Luo, Susannah Gal, Chuan Jian Zhong
DNA-Caged Gold Nanoparticles for Controlled Release of Doxorubicin by a DNA Enzyme and pH Zi-Mou Zhang, Peng-Cheng Gao, Zhi-Fei Wang, Bai-Wang Sun and Yong Jiang* School of Chemistry and Chemical Engineering, Southeast University, Jiangning, Nanjing, Jiangsu, 211189, P. R. China *Corresponding Author Yong Jiang
· In a typical gold nanoparticles/CP assay, the DNA probes were dissolved in a salt solution (112.5 mM NaCl) to form 1.25 μM probe solution at room temperature. Such solution (2.5 μL) was added to gold nanoparticles solution (25 μL), and the obtained solution was incubated for 1 min at room temperature.
Enzymatic DNA processing on gold nanoparticles ... Double stranded DNA immobilised on gold nanoparticles is amenable to enzymatic cleavage by a range of restriction endonucleases and the efficiency of the cleavage can be quantified using fluorescence spectroscopy.
· More information: DNA assembly of nanoparticle superstructures for controlled biological delivery and elimination, Nature Nanotechnology (2014) …
More than 50 years have passed since Clark and Lyon developed the concept of glucose biosensors. Extensive research about biosensors has been carried out up to this day, and an exponential trend in this topic can be observed. The scope of this review is to present various enzyme immobilization methods on gold nanoparticles used for glucose sensing over the past five years.
· You A, Be MAY, In I. Agarose-stabilized gold nanoparticles for surface-enhanced Raman spectroscopic detection of DNA nucleosides. 2015:153114. doi:10.1063/1.2192573 44. Khoobchandani M, Zambre A, Katti K, Lin C, Katti KV.
· Engineered nanoparticles (NPs) open up new horizons to many areas of science and nanotechnology. These engineered nanoparticles gained the attention of many researchers to explore their applications as antibacterial agents, sunscreen, self-cleaning glass, biosensors, paints, electronics, drug delivery tools and many others [1–4].Noble metal NPs such as silver and gold are one of the …
· Multifunctional gold nanoparticle-peptide complexes for nuclear targeting. J Am Chem Soc. 2003;125:4700-1 40. Akhter S, Ahmad MZ, Ahmad FJ, Storm G, Kok RJ. Gold nanoparticles in theranostic oncology: Current state-of-the-art. Expert Opin Drug Deliv. 2012;9:1225-43 41. Murphy CJ, Gole AM, Stone JW, Sisco PN, Alkilany AM, Goldsmith EC. et al.
· Enzymatic manipulation of DNA on well‐dispersed gold nanoparticles (AuNPs) offers an ideal system for high‐throughput screening of G‐quadruplex ligands and evaluation of their selectivity with respect to duplex and quadruplex DNA (see figure). This work has implications for the future use of nanoparticle‐based technologies in the discovery of potential cancer therapeutic agents.
· A label-free, homogeneous aptamer-based sensor strategy was designed for the facile colorimetric detection of small target molecules. The format relied on the target-induced protection of DNA aptamer from the enzymatic digestion and its transduction into a detectable signal through the length-dependent adsorption of single-stranded DNA onto unmodified gold nanoparticles (AuNPs).
· Keywords: DNA methylation, Gold nanoparticles probe, Surface plasmon resonance, Visual detection, Semi-quantitative assay Background DNA methylation is an important regulator of gene expression, and its role in tumorigenesis has been a central topic in the last few decades [ 1 ].
· Enzyme-induced in vivo assembly of gold nanoparticles for imaging-guided synergistic ... the shielding PEG layer was rapidly cleaved to expose the complementary DNA strands on nanoparticle surface. ... nanoplatform may find applications for tumor-targeted imaging and therapy and accelerate the clinical translation of enzyme-responsive ...
· The efficient digestion of nanoparticle assemblies connected by DNA linkers is demonstrated using a number of complementary methods (enzymatic cleavage with EcoRI is shown in the scheme). The efficiency of digestion is dependent on the design of the DNA linker. The ability to manipulate DNA enzymatically is important in the development of hierarchical chemical methods of …
addition, gold nanoparticles can be used to test for a wide variety of analytes by coating the nanoparticles with appropriate ligands. This study focuses on using DNA probes attached to a nanoparticle surface in order to detect a specific DNA sequence. Understanding the application of gold nanoparticles for diagnostics requires both an
Analyst Published on 24 June 2013. Downloaded by Agency for Science, Technology & Research (A*STAR) on 30/07/2013 05:08:35. View Article Online PAPER View Journal | View Issue DNA assembly and enzymatic cutting in solutions: a gold nanoparticle based SERS detection strategy† Cite this: Analyst, 2013, 138, 4941 Elizabeth Crew,a Hong Yan,a Liqin Lin,a Jun Yin,a Zakiya Skeete,a Timur …
Enzyme-Assisted Gold Nanoparticle Amplification XIAOJI XIE, WEI XU, AND XIAOGANG LIU* ... RECEIVED ON FEBRUARY 9, 2012 CONSPECTUS T he discovery of the DNA-mediated assembly of gold nanoparticles was a great moment in the history of science; this ... The aggregation is a reversible process because of the reversible nature of DNA hybridization ...
· In the present study, a novel and facile method is introduced for the sensitive detection of the M.SssI methyltransferase (M.SssI MTase) enzyme and methylated DNA based on the fluorescence recovery of FAM-labeled DNA coupled with gold nanoparticles (AuNPs).
· Probing DNA''s Interstrand Orientation with Gold Nanoparticles. Analytical Chemistry 2011, 83 (13), 5067-5072. DOI: 10.1021/ac200987e. Nishi Bhatt, Po-Jung Jimmy Huang, Neeshma Dave, and Juewen Liu . Dissociation and Degradation of Thiol-Modified DNA on Gold Nanoparticles in Aqueous and Organic Solvents.
· Oct 24, 2011: Gold nanoparticles speed up discovery of the genetic patterns responsible for disease (Nanowerk News) Many illnesses are now being linked to particular patterns of mutations in an individual''s DNA.However, the search for these patterns, known as haplotypes, is generally a slow and painstaking process.
· Ultrasensitive detection of DNA and RNA based on enzyme-free click chemical ligation chain reaction on dispersed gold nanoparticles. Kato D(1), Oishi M. Author information: (1)Division of Materials Science, Faculty of Pure and Applied Sciences, University of Tsukuba, 1-1-1 Tennoudai, Tsukuba, Ibaraki 305-8573, Japan.
